Effect of Thiamine on Serum Glucagon and Reactive Oxygen Species (ROS) in Perioperative Stress Response

Authors

  • Bastian Lubis
    Department of Anesthesiology and Intensive Care, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Aznan Lelo
    Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Sri Rahmadhona
    Department of Anesthesiology and Intensive Care, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Putri Amelia
    Department of Pediatric, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

Abstract

Background: General anesthesia surgery induces a stress response, altering metabolic patterns and increasing glucagon and reactive oxygen species levels. Thiamine is hypothesized to mitigate this response. Methods: This randomized controlled trial employed a pretest-posttest control group design. Thirty patients undergoing surgery at Adam Malik General Hospital were randomized to receive either thiamine or normal saline. Serum glucagon and ROS levels were measured before and after intervention. Data were analyzed using SPSS 25.0 with a significance level of 0.05. Results: Baseline thiamine levels showed no significant difference between groups (p = 0.896). Post-intervention, the thiamine group exhibited significantly higher thiamine levels (8.69 ± 2.185 ng/ml) compared to the control group (4.52 ± 2.185 ng/ml, p = 0.04). ROS levels were significantly higher in the control group (363.2074 ± 103.74 ng/ml) than the thiamine group (343.239 ± 102.05 ng/ml, p = 0.040). Conclusion: Perioperative thiamine administration effectively suppressed the surgical stress response, demonstrated by decreased ROS levels. The higher thiamine levels in the treatment group suggest a potential mechanism for this effect. Further research is warranted to explore the clinical implications of these findings.